reference strain atcc 17978  (ATCC)

Journal: Scientific Reports

Article Title: In vitro assessment of an antimicrobial peptide against Acinetobacter baumannii persister cells

doi: 10.1038/s41598-025-33137-w

Figure Lengend Snippet: Antibacterial and anti-persister activity of the designed AMP. A Broth microdilution assay illustrating the antibacterial activity of the designed antimicrobial peptide and colistin against A. baumannii ATCC 17,978. The top two rows represent the growth control wells (no antimicrobial treatment). The next three rows correspond to serial twofold dilutions of colistin, and the three rows below correspond to serial twofold dilutions of the designed peptide. Wells showing no visible turbidity indicate inhibition of bacterial growth at the respective concentration. B Growth curves of control (no antimicrobial treatment), peptide-treated, and colistin-treated samples over 24 h. Statistical significance vs. control was determined by Tukey’s multiple comparisons test: ** p < 0.01, **** p < 0.0001. Data are presented as mean ± standard deviation (SD) of three independent experiments ( n = 3).

Article Snippet: The reference strain A. baumannii ATCC 17,978, originally isolated from a neonatal meningitis case, was used in this study.

Techniques: Activity Assay, Microdilution Assay, Control, Inhibition, Concentration Assay, Standard Deviation

Journal: Scientific Reports

Article Title: In vitro assessment of an antimicrobial peptide against Acinetobacter baumannii persister cells

doi: 10.1038/s41598-025-33137-w

Figure Lengend Snippet: Representative agar plates showing colony growth of A. baumannii under different treatment conditions. Bacterial survival was evaluated following exposure to the designed AMP and colistin. (A) the untreated control showing dense colony growth, (B) cultures treated with colistin, and (C) cultures treated with the designed peptide. A marked reduction in colony numbers was observed in treated samples compared with the control.

Article Snippet: The reference strain A. baumannii ATCC 17,978, originally isolated from a neonatal meningitis case, was used in this study.

Techniques: Control

Journal: Scientific Reports

Article Title: In vitro assessment of an antimicrobial peptide against Acinetobacter baumannii persister cells

doi: 10.1038/s41598-025-33137-w

Figure Lengend Snippet: Antibacterial activity and cytotoxicity assessment in HT29 epithelial cell cultures. A Colony enumeration of A. baumannii after treatment with the designed peptide at 32 and 64 µg/mL compared with untreated control samples, demonstrating dose-dependent antibacterial effects in infected HT29 cells. Statistical significance vs. control was determined by Tukey’s multiple comparisons test: * p < 0.05. B Cytotoxicity profile of the peptide on HT29 cells at the same concentrations, showing the percentage of viable cells and indicating a tolerable cytotoxicity range.

Article Snippet: The reference strain A. baumannii ATCC 17,978, originally isolated from a neonatal meningitis case, was used in this study.

Techniques: Activity Assay, Control, Infection

Journal: Scientific Reports

Article Title: In vitro assessment of an antimicrobial peptide against Acinetobacter baumannii persister cells

doi: 10.1038/s41598-025-33137-w

Figure Lengend Snippet: Time-dependent inhibitory effect of the designed AMP on A. baumannii biofilm formation. No significant reduction in biofilm formation was observed at early time points (1 h and 4 h). However, treatment with the peptide (64 µg/mL) significantly reduced biofilm biomass after 24 h, as indicated by a decrease in mean OD 550 from 0.71 to 0.49 ( p = 0.0056).

Article Snippet: The reference strain A. baumannii ATCC 17,978, originally isolated from a neonatal meningitis case, was used in this study.

Techniques:

Journal: Scientific Reports

Article Title: In vitro assessment of an antimicrobial peptide against Acinetobacter baumannii persister cells

doi: 10.1038/s41598-025-33137-w

Figure Lengend Snippet: Expression of target genes in A. baumannii following peptide treatment. Relative expression levels of pmr B and las I were measured at 1, 4, and 24 h after exposure to the designed peptide, compared with untreated control samples. 16 S rRNA was used as the internal reference for normalization, and fold changes are presented to illustrate the peptide-induced stress response. Statistical significance was determined by Tukey’s multiple comparisons test: ** p < 0.01, **** p < 0.0001.

Article Snippet: The reference strain A. baumannii ATCC 17,978, originally isolated from a neonatal meningitis case, was used in this study.

Techniques: Expressing, Control